pKG279 - pPB.USI-TRE3G-hNgn2x3HA-WPRE-DSF.CAG-rtTA-2A-EBFP2-bGH

pCF279

• Species: NEB Stable

• Stock date: 2026-10-14

Resistances

• Ampicillin

Plasmid type

• Insertion::PiggyBac

Features

• piggyBac right (3’) inverted repeat

• piggyBac left (5’) inverted repeat

• AmpR promoter

• AmpR

• lac promoter

• CAP binding site

• lac operator

• ori

• f1 ori

• piggybac 3’ ITR

• piggybac 5’ ITR

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• TRE3G promoter

• 3xHA

• Kozak sequence

• AP

• AP

• AP

• AP

• AP

• AP

• AP

• AP

• hNgn2 (codon optimized)

• WPRE

• XbaI

• CMV enhancer

• chicken β-actin promoter

• chimeric intron

• EF-1α promoter

• Tet-On® 3G

• P2A

• Kozak sequence

• bGH poly(A) signal

• EBFP2