pKG900 - PB-EF1a-attBbxb1-EGFPpArev-attPbxb1

pFLIP79

• Species: NEB Stable

• Stock date: 2021-09-14

Resistances

• Ampicillin

Plasmid type

• Viral::Lenti

Features

• Gibson reverse

• Destroy Not1

• Rabit globbin poly A

• mcherry primer annealing

• Gibson reverse

• Gibson forward

• Destroy Not1

• Destroy Mlu1

• Bxb1 attP(r)

• kozak

• Bxb1 attB(f)

• GFP

• CAG promoter

• EF-1α promoter

• pENTR-Gibson-FWD

• pENTR-Gibson-REV

• XbaI

• attL2

• attL1

• EF-1α promoter

• EF-1α intron A

• EF-1α core promoter

• Puro

• SV40 promoter

• SV40 ori

• bGH poly(A) signal

• PuroR

• WPRE

• T3 promoter

• T7 promoter

• f1 ori

• ori

• lac operator

• CAP binding site

• lac promoter

• AmpR

• AmpR promoter

• piggyBac left (5’) inverted repeat

• piggyBac right (3’) inverted repeat

• attB1

• attB2