pKG1238 - pENTR-EF1a-Halo-Trp53-bGH

pCPO29

• Species: NEB Stable

• Stock date: 2022-05-12

Resistances

• Kanamycin

Plasmid type

• Gateway::Entry

Features

• EF-1α intron A

• EF-1α promoter

• rrnB T2 terminator

• rrnB T1 terminator

• attL1

• ori

• pENTR-Gibson-REV

• HaloTag®

• TEV site

• Factor Xa site

• p53_Nterm

• p53_Cterm

• NES

• TET

• NLS

• potential Top1

• TRP53(mouse)

• DNA Binding Domain

• N-Terminus

• C-Terminus

• p53DD-2ndHalf

• PRD

• TAD I

• 4D

• TAD II

• Implied Top1 Interaction Domain

• NLS

• P53DD_1st_Half

• NES

• Target site for sh-p53-2 TRCN0000331409

• target site for sh-p53 1 - TRCN0000310844

• bGH poly(A) signal

• pENTR2B.Seq.REV

• ori

• attL2

• KanR