pKG2685 - LentiX1-USI-TRE3G-H2B-tagBFP-bGH-DSF-hPGK-Halo-p53-WPRE

pAMB170

• Species: NEB Stable

• Stock date: 2023-12-20

Resistances

• Ampicillin

Plasmid type

• Viral::Lenti::3rd gen

Features

• AmpR promoter

• AmpR

• ori

• CAP binding site

• lac promoter

• lac operator

• T3 promoter

• RSV promoter

• 5’ LTR (truncated)

• HIV-1 Psi

• RRE

• cPPT/CTS

• 3’ LTR (Delta-U3)

• SV40 poly(A) signal

• SV40 ori

• T7 promoter

• f1 ori

• TRE3G promoter

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• Kozak sequence

• TagBFP

• H2B

• bGH poly(A) signal

• XbaI

• hPGK promoter

• target site for sh-p53 5 - TRCN00003312189

• target site for sh-p53 1 - TRCN0000310844

• Target site for sh-p53-2 TRCN0000331409

• NES

• P53DD_1st_Half

• NLS

• Implied Top1 Interaction Domain

• TAD II

• 4D

• TAD I

• PRD

• p53DD-2ndHalf

• C-Terminus

• N-Terminus

• DNA Binding Domain

• TRP53(mouse)

• potential Top1

• NLS

• TET

• NES

• p53_Cterm

• p53_Nterm

• Factor Xa site

• TEV site

• HaloTag®

• WPRE