pKG3057 - USI.EF1a.iCre.2A.mRuby2(FF4).TS.FF4x1

pRC273

• Species: NEB Stable

• Stock date: 2024-05-13

Resistances

• Kanamycin

Plasmid type

• Golden Gate::pShip

Features

• pENTR2B.Seq.REV

• KanR

• pBR322_Origin

• ori

• rrnB T1 terminator

• rrnB T2 terminator

• EF-1α promoter

• EF-1α intron A

• EF-1a core promoter

• Kozak sequence

• NLS-iCre

• P2A

• mRuby2

• mRuby2

• Kozak sequence

• 3’ Acceptor Site

• PolyPyrTract

• Branchpoint

• Insert 3

• Mv3 Intron

• Mv3 Intron

• FF4 Hairpin

• miR-FF4 target site

• bGH poly(A) signal