pKG3662 - pShip-EFS-ZF10-NS3-Timsx2-p65-P2A-iRFP720-bGH

pGEEC572

• Species: NEB Stable

• Stock date: 2024-09-21

Resistances

• Kanamycin

Plasmid type

• Golden Gate::pShip

Features

• KanR

• ori

• rrnB T1 terminator

• rrnB T2 terminator

• EF-1α core promoter

• HS249F

• Kozak

• Start

• ZF10-1

• HS251F

• HS250R

• SbfI-HF (for ZF swap)

• GS linker

• HS252R

• NS5A/5B cutsite (CC)

• HS239F

• N-end rule

• AU1

• NS4A

• NS3

• HIS-57, catalytic triad

• Asp 81, catalytic triad

• Ser 139, catalytic triad

• Asp168

• NS4A/4B cutsite (CS)

• Tims from IWS1

• Tims from IWS1

• p65(aa361-551)

• Stop

• iRFP720

• 3xFLAG

• P2A

• HA

• SV40 NLS

• bGH poly(A) signal