Species: NEB stable
Stock date: 2025-05-04
Ampicillin
IVT
AmpR promoter
AmpR
PuroR
ori
T7 promoter
nsP1
opal terminator codon skipped
nsP4
5’ UTR
51nt CSE
subgenomic promoter (SGP)
SG 5’ UTR
3’ UTR
linearize plasmid w/ MluI for IVT
IRES
cleancapAU promoter
nsP2
nsP3’
COX8 presequence
NES
mito-sensor