pKG4382 - pHA.SI-GA-Convergent-v1-VP16-microRNA

pEP161

• Species: NEB Stable

• Stock date: 2025-08-20

Resistances

• Ampicillin

Plasmid type

• Insertion::Recombinase

Features

• AmpR

• ori

• AmpR promoter

• hybrid intron

• chicken β-actin promoter

• CMV enhancer

• CBh

• FRT-F3 mutant

• FRT

• attB

• a>g domesticate PaqCI

• Kozak sequence

• YB TATA_min

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37x6-C

• Kozak sequence

• mRuby2

• SV40 NLS

• 3xFLAG

• VP16-ZF37

• ZF37

• VP16

• P2A

• mGreenLantern

• 3’ miR-30a in pPRIME

• 5’ miR-30a in pPRIME

• 3’ miR-30a

• 5’ miR-30a

• intron

• intron

• FF5 hairpin

• mGreenLantern

• miR-FF6 Target Site

• bGH poly(A) signal

• XbaI

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43_6x_C-promoter

• Kozak sequence

• mRuby2

• P2A

• Kozak sequence

• VP16

• ZF43

• 3xFLAG

• SV40 NLS

• VP16-ZF43

• 5’ miR-30a

• 3’ miR-30a

• 5’ miR-30a in pPRIME

• 3’ miR-30a in pPRIME

• Kozak sequence

• mRuby2

• mRuby2

• FF6 Hairpin

• miR-FF6 Target Site

• miR-FF6 Target Site

• FF5 target site

• bGH poly(A) signal