pKG4579 - pShip-ZF37x6-C-VP16-ZF37-P2A-mGL(miR-FF5)-ZF43-TS.FF6x1-bGH

pEP258

• Species: NEB Stable

• Stock date: 2025-11-19

Resistances

• Kanamycin

Plasmid type

• Golden Gate::pShip

Features

• pENTR2B.Seq.REV

• KanR

• pBR322_Origin

• ori

• rrnB T1 terminator

• rrnB T2 terminator

• YB TATA_min

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37x6-C

• Kozak sequence

• SV40 NLS

• 3xFLAG

• VP16-ZF37

• ZF37

• VP16

• P2A

• SV40 NLS

• 3xFLAG

• mGreenLantern

• mGreenLantern

• ZF43

• 3’ miR-30a in pPRIME

• 5’ miR-30a in pPRIME

• 3’ miR-30a

• 5’ miR-30a

• intron

• intron

• FF5 hairpin

• miR-FF6 Target Site

• bGH poly(A) signal