pKG4911 - pPB-NoSelect-Harbor_long_ITRs-Tet-VP16-combined-repression-separate-mRuby2

pEP420

• Species: NEB Stable

• Stock date: 2026-02-13

Resistances

• Ampicillin

Plasmid type

• Insertion::PiggyBac

Features

• piggyBac right (3’) inverted repeat

• piggyBac left (5’) inverted repeat

• AmpR promoter

• AmpR

• lac promoter

• CAP binding site

• lac operator

• ori

• f1 ori

• piggybac 3’ ITR

• piggybac 5’ ITR

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• YB TATA_min

• Kozak sequence

• P2A

• Kozak sequence

• VP16

• ZF43

• 3xFLAG

• SV40 NLS

• SV40 NLS

• 3xFLAG

• mRuby2

• mRuby2

• ZF37

• 5’ miR-30a

• 3’ miR-30a

• 5’ miR-30a in pPRIME

• 3’ miR-30a in pPRIME

• Kozak sequence

• FF6 Hairpin

• miR-FF6 Target Site

• miR-FF6 Target Site

• FF5 target site

• bGH poly(A) signal