pKG4949 - pSI-GA-USI.CAG-mCherry-JCC030-P2A-Pu.1-bGH.DSF-UbC-EGFP-NanoLuc-WPRE

pCF083

• Species: NEB Stable

• Stock date: 2026-03-04

Resistances

• Ampicillin

Plasmid type

• Golden Gate::Harbor

• Expression

Features

• FRT-F3 mutant

• AmpR

• ori

• f1 ori

• AmpR promoter

• lac promoter

• CAP binding site

• lac operator

• FRT

• EF-1α promoter

• SV40 promoter

• SV40 promoter

• SV40 ori

• attB

• CMV enhancer

• chicken β-actin promoter

• chimeric intron

• EF-1α promoter

• Kozak sequence

• CMV-mCh-gBlock

• splice stem

• splice stem

• xpt guanine ribozyme

• alternative exon

• CMV-mCh-gBlock

• mCherry

• HBB intron

• HBB intron

• Kozak sequence

• mCherry

• P2A

• Pu.1

• bGH poly(A) signal

• XbaI

• UbC promoter

• Kozak sequence

• EGFP

• T2A

• Kozak sequence

• Nluc

• WPRE