pKG5469 - Rogi2-Tet-NFZ-combined-repression-Divergent

pEP459

• Species: NEB Stable

• Stock date: 2026-08-20

Resistances

• Ampicillin

Plasmid type

• Insertion::Recombinase

Features

• AmpR

• ori

• AmpR promoter

• attB

• loxP

• EF-1α promoter

• lox2272

• YB TATA_min

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• Kozak sequence

• 3xFLAG

• VP16-ZF37

• VP16-ZF37

• ZF37

• P2A

• SV40 NLS

• 3xFLAG

• mGreenLantern

• mGreenLantern

• ZF43

• 3’ miR-30a in pPRIME

• 5’ miR-30a in pPRIME

• 3’ miR-30a

• 5’ miR-30a

• intron

• intron

• FF5 hairpin

• SV40 NLS

• NFZ

• miR-FF6 Target Site

• bGH poly(A) signal

• XbaI

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• ZF43 Binding Site

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• tet operator

• YB TATA_min

• Kozak sequence

• P2A

• Kozak sequence

• ZF43

• 3xFLAG

• SV40 NLS

• 3xFLAG

• mRuby2

• mRuby2

• ZF37

• 5’ miR-30a

• 3’ miR-30a

• 5’ miR-30a in pPRIME

• 3’ miR-30a in pPRIME

• Kozak sequence

• FF6 Hairpin

• miR-FF6 Target Site

• miR-FF6 Target Site

• SV40 NLS

• VP16-ZF43

• VP16-ZF43

• ZF43

• NFZ

• FF5 target site

• bGH poly(A) signal