pKG5523 - pSHPN031_USI.EF1a-C-NFZ-ZF43-P2A-mRuby2_miR-FF6_-ZF37-FF6-bGH

pSHPN031

• Species: NEB Stable

• Stock date: 2026-09-14

Resistances

• Kanamycin

Plasmid type

• Golden Gate::pShip

Features

• pENTR2B.Seq.REV

• KanR

• pBR322_Origin

• ori

• rrnB T1 terminator

• rrnB T2 terminator

• EF-1α promoter

• EF-1α intron A

• Kozak sequence

• P2A

• Kozak sequence

• ZF43

• 3xFLAG

• SV40 NLS

• 3xFLAG

• mRuby2

• ZF37

• 5’ miR-30a

• 3’ miR-30a

• 5’ miR-30a in pPRIME

• 3’ miR-30a in pPRIME

• Kozak sequence

• FF6 Hairpin

• miR-FF6 Target Site

• miR-FF6 Target Site

• SV40 NLS

• VP16-ZF43

• ZF43

• NFZ

• miR-FF6 Target Site

• miR-FF6 Target Site

• miR-FF6 Target Site

• miR-FF6 Target Site

• bGH poly(A) signal