pKG5771 - pSHPN049_ZF37x6-C-NFZ-ZF37-P2A-mGL_miR-FF5_-ZF43-FF5-bGH

pSHPN049

• Species: NEB Stable

• Stock date: 2026-10-29

Resistances

• Kanamycin

Plasmid type

• Golden Gate::pShip

Features

• pENTR2B.Seq.REV

• KanR

• pBR322_Origin

• ori

• rrnB T1 terminator

• rrnB T2 terminator

• YB TATA_min

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37

• ZF37x6-C

• Kozak sequence

• 3xFLAG

• VP16-ZF37

• VP16-ZF37

• ZF37

• P2A

• SV40 NLS

• 3xFLAG

• mGreenLantern

• mGreenLantern

• ZF43

• 3’ miR-30a in pPRIME

• 5’ miR-30a in pPRIME

• 3’ miR-30a

• 5’ miR-30a

• intron

• intron

• FF5 hairpin

• SV40 NLS

• NFZ

• FF5 target site

• FF5 target site

• FF5 target site

• FF5 target site

• bGH poly(A) signal

• Golden Gate assembly enzyme: BsaI. Assembly date: 2026-10-14. Construct assembled from: 1. pKG01117; 2. pKG01374; 3. pEP0218; 4. pKG01152; 5. pKG0590