======================== Antibiotic Stock Recipes ======================== 1000X Ampicillin (AMP) ====================== 1. Dissolve 100 mg of Ampicillin powder in 1 mL sterile/ELGA water (Or 1 g of Ampicillin powder in 10 mL sterile/ELGA water) 2. Sterilize with 0.22 uM filter. 3. Make 1 mL aliquots and store at -20C 1000X Kanamycin (KAN) ===================== 1. Dissolve 50 mg of Kanamycin powder in 1 mL sterile/ELGA water (Or 500 mg of Kanamycin powder in 10 mL sterile/ELGA water) 2. Sterilize with 0.22 uM filter. 3. Make 1 mL aliquots and store at -20C 1000X Chloramphenicol (Chlor) ============================= 1. Dissolve 34 mg of Chloramphenicol powder in 1 mL 70% EtOH (Or 340 mg of Chloramphenicol powder in 10 mL 70% EtOH) 2. No filtration step needed. 3. Make 1 mL aliquots and store at -20C.