======================== Antibiotic Stock Recipes ======================== 1000X Ampicillin (Amp) ====================== 1. Dissolve 100 mg of Ampicillin powder in 1 mL sterile/ELGA water (1 g of Ampicillin powder in 10 mL sterile/ELGA water). 2. Sterilize with 0.22 uM filter. 3. Make 1 mL aliquots and store at -20ºC. 1000X Kanamycin (Kan) ===================== 1. Dissolve 50 mg of Kanamycin powder in 1 mL sterile/ELGA water (500 mg of Kanamycin powder in 10 mL sterile/ELGA water). 2. Sterilize with 0.22 uM filter. 3. Make 1 mL aliquots and store at -20ºC. 1000X Chloramphenicol (Chlor) ============================= 1. Dissolve 34 mg of Chloramphenicol powder in 1 mL 70% EtOH (340 mg of Chloramphenicol powder in 10 mL 70% EtOH). 2. No filtration step is needed. 3. Make 1 mL aliquots and store at -20ºC.