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DNA wash buffer
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This wash buffer can be used for the DNA cleanup washes or the second miniprep wash.

DNA wash stock solution (5x)
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Ingredient                  Amount (per 80 mL) 
====================    =============================
80 mM NaCl                  0.374 g
8 mM Tris-HCl               0.101 g
H2O                         80 mL
HCl to pH 7.5
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DNA wash buffer
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Ingredient                  Amount (per 100 mL) 
====================    =============================
5x stock solution           20 mL
Ethanol                     80 mL
====================    =============================

Other Qiagen recipes are available `here <../../_static/files/QiagenBufferRecipes.pdf>`_.