=================== FISH buffer recipes =================== Probe Hybridization Buffer ========================== 1. Combine the following components fresh before use ======================= ============================= Ingredient Amount ======================= ============================= 30% Formamide 3 mL 20X SSC 2.5 mL 1M Citric Acid, pH 6 90 uL 10% Tween 20 100 uL 50X Denhardt's Solution 200 uL 50% Dextran Sulfate 1.5 mL 20 mg/mL BSA 50 uL H20 Bring volume up to 10 mL ======================= ============================= Probe Wash Buffer ================= 1. Combine the following components ==================== ============================= Ingredient Amount ==================== ============================= 30% Formamide 3 mL 20X SSC 2.5 mL 1M Citric Acid, pH 6 90 uL 10% Tween 20 100 uL H20 Bring volume up to 10 mL ==================== ============================= 2. Store at -20 degrees C, expiration in 7 days Probe Amplification Buffer ========================== 1. Combine the following components: ==================== ============================= Ingredient Amount ==================== ============================= Na2HPO4 70.98 mg NaCL 292.2 mg H20 Bring volume up to 10 mL ==================== ============================= 2. Bring the pH to 6.8 (approximately 50 uL of 2.4 M HCl). Store at room temp. 5X SSCT ======= 1. Combine the following components ==================== ============================= Ingredient Amount ==================== ============================= 20X SSC 10 mL 10% Tween 20 400 uL DEPC H20 Bring volume up to 40 mL ==================== ============================= 1. Store at 4 degrees C, expiration in 1 month.