=============== DNA wash buffer =============== This wash buffer can be used for the :doc:`DNA cleanup ` washes or the second :ref:`miniprep ` wash. DNA wash stock solution (5x) ============================ This is stored in a bottle on the shelf above the large thermocyclers. ==================== ============================= Ingredient Amount (per 80 mL) ==================== ============================= 80 mM NaCl 0.374 g 8 mM Tris-HCl 0.101 g Water 80 mL HCl to pH 7.5 ==================== ============================= DNA wash buffer =============== ==================== ============================= Ingredient Amount (per 100 mL) ==================== ============================= 5x stock solution 20 mL Ethanol 80 mL ==================== ============================= Other Qiagen recipes are available `here <../../_static/files/QiagenBufferRecipes.pdf>`_.