D>gt or e
D>gt or e appears in 159 plasmids under 35 names.
+1 Site
-1
A
A->G
A>C silent mutation
a>g domesticate PaqCI
attB
attR2
c>g
c>g for BsaI GG simulation
C>G for GGA
pKG4597: pPB.bistable-VP16-combined-repression-separate-mRuby2
pKG4595: pPB.bistable-VP16-protein-repression-separate-mRuby2
pKG4591: pPB.bistable-VP16-combined-repression-Antisense-Tandem
pKG4562: (DO NOT USE)PB.HaborPaqCI-CAG.d-PuroR-T2A-mTagBFP2-NLS-WPRE
pKG4550: (DO NOT USE)PB.HaborPaqCI-CAG.d-rtTA-P2A-PuroR-T2A-mTagBFP2-NLS-WPRE
C>G for PaqCI GG
Destroy Mlu1
EcoRI
edited to remove EcoRI
EF-1-alpha core promoter
EF-1α promoter
H863A
Kozak sequence
pKG1830: pTET46_Divergent_6xZF43-mGL-bGH-1x.spacer-EF1a-ZF43-TagBFP-bGH
pKG5393: pLXPN002.2_pLENTIX.USI-Tre3G_3xFlag_mRuby2_bGH.DSF-EFS-rtTA-TagBFP-WPRE
pKG5440: pLXPN004.1_pLENTIX.USI-TRE3G-USI-TREG3G-ALFA-mRuby-FF6-bGH.DSF-EFS-rtTA-TagBFP-WPRE
pKG5439: pLXPN004_pLENTIX.USI-TRE3G-USI-TREG3G-ALFA-mRuby2-FF4-bGH.DSF-EFS-rtTA-TagBFP-WPRE
pKG5437: pLXPN002.1_pLENTIX.USI-Tre3G_3xFlag_mRuby2_ff6_bGH.DSF-EFS-rtTA-TagBFP-WPRE
pKG5436: pLXPN002_pLENTIX.USI-Tre3G_3xFlag_mRuby2_ff4_bGH.DSF-EFS-rtTA-TagBFP-WPRE
KpnI 3’ site
New Feature(20)
NLS-iCre
Oct4
possible T (clone 1)
PuroR
removed BamHI site
Rogi2-HA-up
Sal1
silent mutation to remove xhoi
Starting G nucleotide
T in sequencing
T in sequencing, still Leu
T->C
tight TRE promoter
β-globin 5’ UTR