D>gt or
D>gt or appears in 152 plasmids under 35 names.
+1 Site
-1
A
A->G
A>C silent mutation
a>g domesticate PaqCI
attB
attR2
c>g
c>g for BsaI GG simulation
C>G for GGA
pKG4597: pPB.bistable-VP16-combined-repression-separate-mRuby2
pKG4595: pPB.bistable-VP16-protein-repression-separate-mRuby2
pKG4591: pPB.bistable-VP16-combined-repression-Antisense-Tandem
pKG4562: (DO NOT USE)PB.HaborPaqCI-CAG.d-PuroR-T2A-mTagBFP2-NLS-WPRE
pKG4550: (DO NOT USE)PB.HaborPaqCI-CAG.d-rtTA-P2A-PuroR-T2A-mTagBFP2-NLS-WPRE
C>G for PaqCI GG
Destroy Mlu1
EcoRI
edited to remove EcoRI
EF-1-alpha core promoter
EF-1α promoter
H863A
Kozak sequence
KpnI 3’ site
New Feature(20)
NLS-iCre
Oct4
possible T (clone 1)
PuroR
removed BamHI site
Rogi2-HA-up
Sal1
silent mutation to remove xhoi
Starting G nucleotide
T in sequencing
T in sequencing, still Leu
T->C
tight TRE promoter
β-globin 5’ UTR