Antibiotic Stock Recipes
1000X Ampicillin (Amp)
Dissolve 100 mg of Ampicillin powder in 1 mL sterile/ELGA water (1 g of Ampicillin powder in 10 mL sterile/ELGA water).
Sterilize with 0.22 uM filter.
Make 1 mL aliquots and store at -20ºC.
1000X Kanamycin (Kan)
Dissolve 50 mg of Kanamycin powder in 1 mL sterile/ELGA water (500 mg of Kanamycin powder in 10 mL sterile/ELGA water).
Sterilize with 0.22 uM filter.
Make 1 mL aliquots and store at -20ºC.
1000X Chloramphenicol (Chlor)
Dissolve 34 mg of Chloramphenicol powder in 1 mL 70% EtOH (340 mg of Chloramphenicol powder in 10 mL 70% EtOH).
No filtration step is needed.
Make 1 mL aliquots and store at -20ºC.