FISH buffer recipes
Probe Hybridization Buffer
Combine the following components fresh before use
Ingredient |
Amount |
---|---|
30% Formamide |
3 mL |
20X SSC |
2.5 mL |
1M Citric Acid, pH 6 |
90 uL |
10% Tween 20 |
100 uL |
50X Denhardt’s Solution |
200 uL |
50% Dextran Sulfate |
1.5 mL |
20 mg/mL BSA |
50 uL |
H20 |
Bring volume up to 10 mL |
Probe Wash Buffer
Combine the following components
Ingredient |
Amount |
---|---|
30% Formamide |
3 mL |
20X SSC |
2.5 mL |
1M Citric Acid, pH 6 |
90 uL |
10% Tween 20 |
100 uL |
H20 |
Bring volume up to 10 mL |
Store at -20 degrees C, expiration in 7 days
Probe Amplification Buffer
Combine the following components:
Ingredient |
Amount |
---|---|
Na2HPO4 |
70.98 mg |
NaCL |
292.2 mg |
H20 |
Bring volume up to 10 mL |
Bring the pH to 6.8 (approximately 50 uL of 2.4 M HCl). Store at room temp.
5X SSCT
Combine the following components
Ingredient |
Amount |
---|---|
20X SSC |
10 mL |
10% Tween 20 |
400 uL |
DEPC H20 |
Bring volume up to 40 mL |
Store at 4 degrees C, expiration in 1 month.