Glycerol Stocking

After successfully sequencing a bacterial plasmid, we must stock it for storage.

1. Add pKG # to Quartzy database. Add in a new item to the plasmid database, with the criteria below. The pKG number is XXXXX, use the next pKG number available.

Criteria	Label
Item Name	pKGXXXXX (put leading zero if neccesary)
Type	bacterial stock
Date Stored	date of storage
Plasmid	description of construct (ex. LentiX-EF1a-mGL-BGH)
pKG#	XXXXX
Plasmid type	Selection from options
Resistance markers	Selection from options
Species	Bacterial cell type (ex. NEB Stable)

2. Culture desired 4 mL of bacteria clone with the plasmid in LB Broth with the correct resistance antibiotic (overnight).

3. Add 2 mL of 60% glycerol to the 4 mL of culture. Mix well.

4. Aliquot 2 mL of the resulting mixture to three cryovials.

5. Label each cryovial with pKG#, date, initials, and description of the construct.

6. Put cyrovials in -80 C. Put one cryovial per box.