DNA wash buffer

This wash buffer can be used for the DNA cleanup washes or the second miniprep wash.

DNA wash stock solution (5x)

Ingredient

Amount (per 80 mL)

80 mM NaCl

0.374 g

8 mM Tris-HCl

0.101 g

H2O

80 mL

HCl to pH 7.5

DNA wash buffer

Ingredient

Amount (per 100 mL)

5x stock solution

20 mL

Ethanol

80 mL

Other Qiagen recipes are available here.