Small molecule stocks for TC

Indole-3-acetic acid (Auxin, IAA)

  1. Resuspend IAA in ethanol at a ratio of approximately 11 uL ethanol to 2 mg IAA. Vortex until dissolved.

  2. Add mixture to ELGA water to achieve a final IAA concentration of 1.75 mg/mL (10mM).

  3. Sterilize with 0.22 uM filter.

  4. Separate into 50 uL aliquots and store at -20°C.

Indole-3-acetamide (Auxin precursor, IAM)

  1. Resuspend IAM in ethanol at a ratio of approximately 20 uL ethanol to 1 mg IAM.

  2. Add mixture to ELGA water to achieve a final IAM concentration of 1.75 mg/mL (10mM).

  3. Sterilize with 0.22 uM filter.

  4. Separate into 50 uL aliquots and store at -20°C.

50mM O6-Benzylguanine (O6BG)

  1. Dissolve 12 mg of O6BG in 1ml of DMSO.

  2. Sterilize with 0.22 uM filter.

  3. Separate into 50 uL aliquots and store at -20°C.

25mM Guanine

  1. Dissolve 7.5 mg of guanine in 2 ml of 0.2N NaOH/Water. (Guanine can not be dissolved with DMSO/Water)

  2. Sterilize with 0.22 uM filter.

  3. Separate into 50 uL aliquots and store at -20°C.