Small molecule stocks for TC
Indole-3-acetic acid (Auxin, IAA)
Resuspend IAA in ethanol at a ratio of approximately 11 uL ethanol to 2 mg IAA. Vortex until dissolved.
Add mixture to ELGA water to achieve a final IAA concentration of 1.75 mg/mL (10mM).
Sterilize with 0.22 uM filter.
Separate into 50 uL aliquots and store at -20°C.
Indole-3-acetamide (Auxin precursor, IAM)
Resuspend IAM in ethanol at a ratio of approximately 20 uL ethanol to 1 mg IAM.
Add mixture to ELGA water to achieve a final IAM concentration of 1.75 mg/mL (10mM).
Sterilize with 0.22 uM filter.
Separate into 50 uL aliquots and store at -20°C.
50mM O6-Benzylguanine (O6BG)
Dissolve 12 mg of O6BG in 1ml of DMSO.
Sterilize with 0.22 uM filter.
Separate into 50 uL aliquots and store at -20°C.
25mM Guanine
Dissolve 7.5 mg of guanine in 2 ml of 0.2N NaOH/Water. (Guanine can not be dissolved with DMSO/Water)
Sterilize with 0.22 uM filter.
Separate into 50 uL aliquots and store at -20°C.