Antibiotic Stock Recipes
1000X Ampicillin (AMP)
Dissolve 100 mg of Ampicillin powder in 1 mL sterile/ELGA water (Or 1 g of Ampicillin powder in 10 mL sterile/ELGA water)
Sterilize with 0.22 uM filter.
Make 1 mL aliquots and store at -20C
1000X Kanamycin (KAN)
Dissolve 50 mg of Kanamycin powder in 1 mL sterile/ELGA water (Or 500 mg of Kanamycin powder in 10 mL sterile/ELGA water)
Sterilize with 0.22 uM filter.
Make 1 mL aliquots and store at -20C
1000X Chloramphenicol (Chlor)
Dissolve 34 mg of Chloramphenicol powder in 1 mL 70% EtOH (Or 340 mg of Chloramphenicol powder in 10 mL 70% EtOH)
No filtration step needed.
Make 1 mL aliquots and store at -20C.