Antibiotic Stock Recipes

1000X Ampicillin (Amp)

  1. Dissolve 100 mg of Ampicillin powder in 1 mL sterile/ELGA water (1 g of Ampicillin powder in 10 mL sterile/ELGA water).

  2. Sterilize with 0.22 uM filter.

  3. Make 1 mL aliquots and store at -20ºC.

1000X Kanamycin (Kan)

  1. Dissolve 50 mg of Kanamycin powder in 1 mL sterile/ELGA water (500 mg of Kanamycin powder in 10 mL sterile/ELGA water).

  2. Sterilize with 0.22 uM filter.

  3. Make 1 mL aliquots and store at -20ºC.

1000X Chloramphenicol (Chlor)

  1. Dissolve 34 mg of Chloramphenicol powder in 1 mL 70% EtOH (340 mg of Chloramphenicol powder in 10 mL 70% EtOH).

  2. No filtration step is needed.

  3. Make 1 mL aliquots and store at -20ºC.