Protocols
- Biochemical and analytical protocols
- Homemade EU click
- HIVE scRNA-seq
- Immunofluorescent Staining
- Alkaline Phosphatase Staining
- ATAC-see
- CellBaum User’s Guide
- CellTrace Dye Labeling
- Sample Prep and Confocal Imaging at the Koch Microscopy core
- HCR DNA-FISH
- Halo-tag Labeling
- HCR RNA-FISH
- Live nuclear staining using Hoechst
- Image segmentation and particle analysis in ImageJ
- KAPA SYBR® FAST qPCR
- Image analysis using the KI workstation - Nemo
- Magnetic bead clean-up for genomics libraries
- Nuclei Isolation for Downstream Genomics Applications
- Psoralen-qPCR supercoiling assay
- sci-ATAC-seq
- Single cell qPCR
- SingleShot RNA isolation and Reverse Transcription
- Snap-tag Labeling
- Titration of Tn5 transposition in MEFs
- Western Blot
- Cloning protocols
- in vitro transcription
- Design and preparation of DNA IVT template
- Creating a new IVT template plasmid
- Generating IVT linear DNA template from an IVT template plasmid
- Creating a new IVT template plasmid - NEED TO UPDATE
- modRNA synthesis
- IVT reaction
- MluI digestion for pKG3198 (polyA encoded on plasmid)
- IVT using MluI-digested pKG3198 (polyA encoded on plasmid)
- mRNA transfection
- MessengerMax transfection protocol
- StemMACS transfection protocol
- Protein production
- TC protocols