Protocols
- Biochemical and analytical protocols
- Genomics and sequencing methods
- Homemade EU click
- Bulk RNAseq with Plasmidsaurus
- Immunofluorescent Staining
- Alkaline Phosphatase Staining
- ATAC-see
- CellBaum User’s Guide
- CellTrace Dye Labeling
- Sample Prep and Confocal Imaging at the Koch Microscopy core
- Droplet Digital PCR (ddPCR)
- HCR DNA-FISH
- Halo-tag Labeling
- HCR RNA-FISH
- Live nuclear staining using Hoechst
- Image segmentation and particle analysis in ImageJ
- KAPA SYBR® FAST qPCR
- Image analysis using the KI workstation - Nemo
- Magnetic bead clean-up for genomics libraries
- Nuclei Isolation for Downstream Genomics Applications
- Psoralen-qPCR supercoiling assay
- Single cell qPCR
- SingleShot RNA isolation and Reverse Transcription
- Snap-tag Labeling
- Western Blot
- Cloning protocols
- Cloning Workflow Overview
- Cloning Workflow Timeline
- Colony PCR
- Restriction digest
- Gel electrophoresis and extraction of DNA
- Gibson assembly
- Glycerol Stocking
- Golden Gate assembly
- Ligation assembly
- LR cloning
- Oligo annealing for ligation cloning
- PCR
- Plasmid Prepping
- Cloning tips, tricks, and troubleshooting
- Touchdown PCR
- Bacterial Transformation
- in vitro transcription
- Protein production
- TC protocols