Stale warning

This protocol has not been updated this year! Last update was on January 26, 2024.

FISH buffer recipes

Probe Hybridization Buffer

1. Combine the following components fresh before use

Ingredient	Amount
30% Formamide	3 mL
20X SSC	2.5 mL
1M Citric Acid, pH 6	90 uL
10% Tween 20	100 uL
50X Denhardt’s Solution	200 uL
50% Dextran Sulfate	1.5 mL
20 mg/mL BSA	50 uL
H20	Bring volume up to 10 mL

Probe Wash Buffer

1. Combine the following components

Ingredient	Amount
30% Formamide	3 mL
20X SSC	2.5 mL
1M Citric Acid, pH 6	90 uL
10% Tween 20	100 uL
H20	Bring volume up to 10 mL

2. Store at -20 degrees C, expiration in 7 days

Probe Amplification Buffer

1. Combine the following components:

Ingredient	Amount
Na2HPO4	70.98 mg
NaCL	292.2 mg
H20	Bring volume up to 10 mL

2. Bring the pH to 6.8 (approximately 50 uL of 2.4 M HCl). Store at room temp.

5X SSCT

1. Combine the following components

Ingredient	Amount
20X SSC	10 mL
10% Tween 20	400 uL
DEPC H20	Bring volume up to 40 mL

1. Store at 4 degrees C, expiration in 1 month.