FISH buffer recipes

Probe Hybridization Buffer

1. Combine the following components fresh before use

Ingredient	Amount
30% Formamide	3 mL
20X SSC	2.5 mL
1M Citric Acid, pH 6	90 uL
10% Tween 20	100 uL
50X Denhardt’s Solution	200 uL
50% Dextran Sulfate	1.5 mL
20 mg/mL BSA	50 uL
H20	Bring volume up to 10 mL

Probe Wash Buffer

1. Combine the following components

Ingredient	Amount
30% Formamide	3 mL
20X SSC	2.5 mL
1M Citric Acid, pH 6	90 uL
10% Tween 20	100 uL
H20	Bring volume up to 10 mL

2. Store at -20 degrees C, expiration in 7 days

Probe Amplification Buffer

1. Combine the following components:

Ingredient	Amount
Na2HPO4	70.98 mg
NaCL	292.2 mg
H20	Bring volume up to 10 mL

2. Bring the pH to 6.8 (approximately 50 uL of 2.4 M HCl). Store at room temp.

5X SSCT

1. Combine the following components

Ingredient	Amount
20X SSC	10 mL
10% Tween 20	400 uL
DEPC H20	Bring volume up to 40 mL

1. Store at 4 degrees C, expiration in 1 month.