TC recipes

• DNase (2X) and papain (2X)
  • DNase and papain from 2X
  • L-Cysteine (50 mM)
  • Papain (2X: 1mM EDTA, 2mM L-cysteine, 40 U papain/mL)
  • DNase (2X: 4,000 U/mL solution)
• FBS heat inactivation
• Common aliquot sizes for TC
  • Neurotrophic factor stocks (10kx and 1kx)
  • RepSox (1kx)
  • Fetal Bovine Serum (FBS) Aliquots (50 mL)
  • Pen-strep aliquots (100X, 5 mL)
  • 50X Geltrex aliquots
• TC media recipes
  • N3 media
  • MEF media
  • Breast cancer 1 media
  • Breast cancer 2 media
  • Breast cancer 3 media
  • Breast cancer 4 media
  • Epithelial cell 1 media
  • Epithelial cell 2 media
  • Glia media
  • Sorting/Collection media
  • Motor neuron dissociation media
  • Freezing media
  • HEPES-buffered DMEM
  • iPSC Reprogramming Media
• Other TC Solutions
  • Phosphate buffered saline (PBS)
  • 0.1% Gelatin
  • EDTA (0.5M, pH=8.0)
• PEI Stocks
  • Materials
  • Dissolve and Aliquot PEI
  • Test PEI Ratio
  • Reference
• Small molecule stocks for TC
  • Antibiotic concentrations
  • Other stock and working concentrations
  • Indole-3-acetic acid (Auxin, IAA)
  • Indole-3-acetamide (Auxin precursor, IAM)
  • 50mM O6-Benzylguanine (O6BG)
  • 25mM Guanine
  • 50 μM AP1903/Rimiducid (1000x stock - old DSP)
  • 1 mM or 100 µM AP1903/Rimiducid (100kx or 10kx stock - current NBW)
  • Puromycin, 1 mg / mL (1,000x stock)