DNA wash buffer
This wash buffer can be used for the DNA cleanup washes or the second miniprep wash.
DNA wash stock solution (5x)
Ingredient |
Amount (per 80 mL) |
|---|---|
80 mM NaCl |
0.374 g |
8 mM Tris-HCl |
0.101 g |
H2O |
80 mL |
HCl to pH 7.5 |
DNA wash buffer
Ingredient |
Amount (per 100 mL) |
|---|---|
5x stock solution |
20 mL |
Ethanol |
80 mL |
Other Qiagen recipes are available here.