Stale warning
This protocol has not been updated this year! Last update was on June 09, 2023.
DNA wash buffer
This wash buffer can be used for the DNA cleanup washes or the second miniprep wash.
DNA wash stock solution (5x)
Ingredient |
Amount (per 80 mL) |
|---|---|
80 mM NaCl |
0.374 g |
8 mM Tris-HCl |
0.101 g |
H2O |
80 mL |
HCl to pH 7.5 |
DNA wash buffer
Ingredient |
Amount (per 100 mL) |
|---|---|
5x stock solution |
20 mL |
Ethanol |
80 mL |
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